Directed Evolution of Split APEX2 Peroxidase
نویسندگان
چکیده
منابع مشابه
Directed evolution of APEX2 for electron microscopy and proteomics
Here we discuss our additional efforts to understand why the A134P mutation in APEX2 renders it far more active than APEX inside cells. In the main text, we described how the most striking difference we could detect between APEX and APEX2 was their varying susceptibilities to H 2 O 2-induced inhibition. In the presence of 1.4 mM of the model phenolic substrate guaiacol, APEX displayed severe in...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2019
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.8b00919